Introduction of Common Service
The Common Service of Chemical Analysis for Ecology (CS CAE) of IMBE is located on the campus de l’étoile (St Jérôme). It was crated in 2018 in order to bring a technical support and logistic to researchers for biological-chemistry cross-disciplinary analysis. This service offers particularly analysis in order to estimate water and ground quality for answering at environmental questions ( effect of human-made of media, effect of stress to the environment).
Members
Staff | Function | Speciality | Localisation |
Loris CAGNACCI | AI IRD | Biotechnology, Molecular and Cellular Biology, Microbiology | St Jérôme - 431 |
Lisa FOLI | TCN AMU | Microbiology | St Jérôme - 441 |
Martine MARTINEZ | AJT IRD | Microbiology | St Jérôme - 461 |
Services
Offered services :
• Advice in sampling, in samples preparation and analytical protocol
• Provided equipment for analysis
• Training for analytical equipment.
• Scientific monitoring, methods development and optimization linked with research programs/themes
• Preliminary assistance for setting scientific projects up and responses to request for proposals (technical feasibility, costs, etc.).
Equipements/Laboratory
Storage
Cold Room (+ 4°C), service 431
Sampling
Freeze Dryer, service 431
Grinding (knifes and balls bearing), service TP D41
Autoclave, service TP D41
Microbiology
Microbiology Safety Cabinet, service 441
Microorganisms laboratory and media preparation, service 441
Analytical Equipment
Ionic Chromatography (IC)
– brand & model : Metrohm 930 Compact IC Flex
– detector : conductimeter
– running analysis : anionic quantification (F-, Cl-, NO2-, NO3-, PO43-, SO42-), cationic quantification (Na+, NH4+, K+, Ca2+, Mg2+) and dissolved silica in liquid samples
– analyzed matrix : filtered freshwater or brackish water, filtered leachate, aqueous extract…
localisation : service 431
High Performation Liquid Chromatography (HPLC)
– brand & model : Agilent 1200 series
– detectors : DAD, fluorescence, refractive index
localisation : service 431
Elemental Analyser
– brand & model : ThermoScientific FlashEA 1112
– dectector : katharometer (TCD)
– running analysis : carbon and nitrogen quantification
– analyzed matrix : ground, sediment, plants, litter, …
localisation : service 431
Gas Chromatography (GC)
– brand & model : Agilent 8860
– dectector : katharometer (TCD)
– running analysis : respirometry
– analyzed matrix : gas
localisation : service 441
UV-Visible Spectrophotometer double beam
– brand & model : Jasco V 630
– running analysis : chlorophylle quantification
– analyzed matrix : liquids
localisation : service 431
Guideline
Conditions of CS CAE use
The form has to be fill in and return to ace@imbe.fr
Request Form
The form has to be fill in and return to ace@imbe.fr
1- Staff request and analytical tools for research project
In advance of submitting project, contact skilled staff in the field in order to plan your experimentation (sample number, sampling date or period, samples preparation and analysis) as well as an analytical estimate.
If the project is hold, let involved staff know and confirm sampling date or period, samples preparation and analysis.
2- Analysis Request
If possible, before doing your sampling (some has to be immediately treated while others can be conserved), contact the technical head in order to ask his availability as well as the equipment ones and analysis delay.
Samples preparations are made by the requester (filtration, conditioning, drying, …) and only the analysis will be done by CS CAE staff. Imperatively fill the request form in otherwise analysis will not be done. While analysis returned, CS CAE will give a period of 4 weeks at the requestor to verify his/her data. Over the deadline, CS CAE will flush aliquots out.
3- Using CS CAE’s analytical tools by a trained scientist
Only a trained and skilled scientist by the responsible for the equipment will be able to work in full autonomy.
Booked the machine by subscribing on the notebook near the machine.
The D-day, you can run your analysis by following the hygiene and safety rules of laboratory.
Do not forget to fill the tracking sheet (number of analysis, parameters, running time, …).
And do not forget to clean the machine and laboratory after you.
4- Analytical cost
CS CAE budget is indispensable for machines’ maintenance. Basic consumables (gloves, tips, diluent, acids, basic media, …) are furnished.
Only specific consumables are in charge of the requester (tin basket, specific column, specific media for culture,). A more important financial support can be requested if a big number of samples are analyzed.
Punctual financial support can be asked in case of important breakdown of machine.
5- Sign of recognition of CS CAE’s involvement in samples analysis and/or project research
In function of the CS CAE’s involvement and the workload request to the staff. CS CAE will ask sign of recognition from requesters either by naming CS CAE in acknowledgments or by including staff as co-author.