{"id":9553,"date":"2025-11-21T11:27:16","date_gmt":"2025-11-21T10:27:16","guid":{"rendered":"https:\/\/www.imbe.fr\/?post_type=tribe_events&#038;p=9553"},"modified":"2025-11-21T11:27:16","modified_gmt":"2025-11-21T10:27:16","slug":"these-de-breaud-celia-nouvelle-strategie-dans-la-recherche-dagents-antituberculeux-naturels-issus-de-la-flore-de-guyane-francaise","status":"publish","type":"tribe_events","link":"https:\/\/www.imbe.fr\/en\/evenement\/these-de-breaud-celia-nouvelle-strategie-dans-la-recherche-dagents-antituberculeux-naturels-issus-de-la-flore-de-guyane-francaise\/","title":{"rendered":"BREAUD C\u00e9lia's thesis \u201cNew strategy in the search for natural anti-tuberculosis agents derived from the flora of French Guyana\u201d.\u201d"},"content":{"rendered":"<p>Mrs.<a href=\"https:\/\/www.imbe.fr\/en\/annuaire\/celia-breaud\/\"> <strong>BREAUD C\u00e9lia<\/strong><\/a><strong>,<\/strong> doctoral student at IMBE, team <a href=\"https:\/\/www.imbe.fr\/en\/recherche\/equipes-de-recherche\/ecosom-ecologie-fonctionnelle-des-systemes-socioecologiques-aux-molecules\/\">Functional ecology: from socio-ecological systems to molecules<\/a>, <strong>will publicly defend his thesis on Monday 15 December 2025 at 2pm in the Thesis Room of the Faculty of Pharmacy.<\/strong><\/p>\n<p><strong>In front of a jury made up of :<\/strong><\/p>\n<ul>\n<li><strong>Sophie TOMASI<\/strong>,Rapporteur and Chair of the Jury, Professor, University of Rennes<\/li>\n<li><strong>Mehdi BENIDDIR<\/strong>, Professor, University of Paris-Saclay<\/li>\n<li><strong>Ga\u00ebtan HERBETTE<\/strong>, Examiner, Research Engineer, Universit\u00e9 Paris-Saclay<\/li>\n<li><strong>St\u00e9phane CANAAN<\/strong>,Examiner, Research Director, University of Aix-Marseille<\/li>\n<li><strong>Sok-Siya BUN-LLOPET<\/strong>, PhD supervisor, University Professor - Hospital Practitioner, University of Aix-Marseille<\/li>\n<li><strong>Elnur GARAYEV<\/strong>, PhD thesis co-director, Senior Lecturer, University of Aix-Marseille<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Summary of work:<\/strong><\/p>\n<p>Tuberculosis remains a major public health issue, reinforced by the emergence of resistant strains. In this context, this thesis explores the flora of French Guyana as a source of metabolites with anti-tuberculosis potential, combining biological screening, metabolomics by LC-MS\/MS and molecular network analysis, with integration of activity data.<\/p>\n<p>The first phase focused on seven species selected on the basis of ethnopharmacological and phytochemical criteria. The extracts and fractions were tested against <em>Mycobacterium tuberculosis<\/em> H37Ra, then profiled by LC-HRMS\/MS. Molecular networks were used to link chemical families and activity levels, prioritise candidates and rapidly dereplicate known compounds, while revealing anti-tuberculosis compounds in several active extracts.<\/p>\n<p>The second part focused on two species of the genus <em>Aspidosperma (<\/em>Apocynaceae), rich in monoterpene indole alkaloids. Bioguided fractionation, coupled with molecular networks, revealed a core of compounds correlated with the inhibition of <em>M. tuberculosis<\/em>. The results support the interest of this chemical reservoir, while highlighting the need for targeted isolations and structural validation by NMR to finely attribute the activity.<\/p>\n<p>The third part dealt with <em>Lantana trifolia<\/em> (Verbenaceae). Fractions of intermediate polarity were found to be the most active, and metabolomic analysis indicated the possible contribution of a small group of abundant metabolites to activity. Global annotation also confirmed a profile rich in flavonoids, phenolic acids and glycosylated phenylpropanoids, suggesting a cumulative or synergistic effect consistent with the distribution of activity observed.<\/p>\n<p>Methodologically, the thesis demonstrates the value of a reproducible pipeline combining high-resolution LC-MS\/MS, automated dereplication and bioactive molecular networks to rationalise the prioritisation of fractions and guide isolation. From a scientific point of view, it documents the chemodiversity of as yet little-studied Amazonian species, establishes clear links between chemical families and antimycobacterial response, and highlights priority molecular groups.<\/p>\n<p>Taken together, this work demonstrates the contribution of integrated metabolomic approaches to exploiting tropical bioresources in the fight against Mycobacteium tuberculosis. They provide a body of analytical and biological data that can be directly mobilised for targeted isolation, pharmacological evaluation and local resource development, in line with an ethical and partnership-based approach to bioprospecting in French Guiana.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>Madame BREAUD C\u00e9lia, doctorant \u00e0 l\u2019IMBE, \u00e9quipe Ecologie fonctionnelle : des syst\u00e8mes socio\u00e9cologiques aux mol\u00e9cules, soutiendra publiquement ses travaux de th\u00e8se le lundi 15 d\u00e9cembre 2025, \u00e0 14h dans la [&hellip;]<\/p>\n","protected":false},"author":300,"featured_media":0,"template":"","meta":{"_acf_changed":false,"_tribe_events_status":"","_tribe_events_status_reason":"","footnotes":""},"tags":[],"tribe_events_cat":[30],"class_list":["post-9553","tribe_events","type-tribe_events","status-publish","hentry","tribe_events_cat-soutenance","cat_soutenance"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/tribe_events\/9553","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/tribe_events"}],"about":[{"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/types\/tribe_events"}],"author":[{"embeddable":true,"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/users\/300"}],"version-history":[{"count":1,"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/tribe_events\/9553\/revisions"}],"predecessor-version":[{"id":9554,"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/tribe_events\/9553\/revisions\/9554"}],"wp:attachment":[{"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/media?parent=9553"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/tags?post=9553"},{"taxonomy":"tribe_events_cat","embeddable":true,"href":"https:\/\/www.imbe.fr\/en\/wp-json\/wp\/v2\/tribe_events_cat?post=9553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}