Common Service of Chemical Analysis for Ecology

Technical Head: Loris Cagnacci
Scientific Head: Isabelle Bombarda

Introduction of Common Service

The Common Service of Chemical Analysis for Ecology (CS CAE) of IMBE is located on the campus de l’étoile (St Jérôme). It was crated in 2018 in order to bring a technical support and logistic to researchers for biological-chemistry cross-disciplinary analysis. This service offers particularly analysis in order to estimate water and ground quality for answering at environmental questions ( effect of human-made of media, effect of stress to the environment).


Members

Staff Function Speciality Localisation
Loris CAGNACCI AI IRD Biotechnology, Molecular and Cellular Biology, Microbiology St Jérôme - 431
Lisa FOLI TCN AMU Microbiology St Jérôme - 441
Martine MARTINEZ AJT IRD Microbiology St Jérôme - 461

Services

Offered services:

• Advice in sampling, in samples preparation and analytical protocol
• Provided equipment for analysis
• Training for analytical equipment.
• Scientific monitoring, methods development and optimization linked with research programs/themes
• Preliminary assistance for setting scientific projects up and responses to request for proposals (technical feasibility, costs, etc.).

Equipements/Laboratory

Storage
Cold Room (+ 4°C), service 431

Sampling
Freeze Dryer, service 431
Grinding (knifes and balls bearing), service TP D41
Autoclave, service TP D41

Microbiology
Microbiology Safety Cabinet, service 441
Microorganisms laboratory and media preparation, service 441

Analytical Equipment

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Ionic Chromatography (IC)
 brand & model: Metrohm 930 Compact IC Flex
 detector: conductimeter
 running analysis: anionic quantification (F-, Cl-, NO2-, NO3-, PO43-, SO42-), cationic quantification (Na+, NH4+, K+, Ca2+, Mg2+) and dissolved silica in liquid samples
 analyzed matrix: filtered freshwater or brackish water, filtered leachate, aqueous extract…
localisation: service 431

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High Performation Liquid Chromatography (HPLC)
 brand & model: Agilent 1200 series
 detectors: DAD, fluorescence, refractive index
localisation: service 431

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Elemental Analyser
 brand & model : ThermoScientific FlashEA 1112
 dectector: katharometer (TCD)
 running analysis: carbon and nitrogen quantification
 analyzed matrix: ground, sediment, plants, litter, …
localisation: service 431

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Gas Chromatography (GC)
 brand & model: Agilent 8860
 dectector: katharometer (TCD)
 running analysis: respirometry
 analyzed matrix: gas
localisation: service 441

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UV-Visible Spectrophotometer double beam
 brand & model: Jasco V 630
 running analysis: chlorophylle quantification
 analyzed matrix: liquids
localisation: service 431

Guideline

Conditions of CS CAE use
The form has to be fill in and return to ace@imbe.fr
Request Form
The form has to be fill in and return to ace@imbe.fr

1- Staff request and analytical tools for research project
In advance of submitting project, contact skilled staff in the field in order to plan your experimentation (sample number, sampling date or period, samples preparation and analysis) as well as an analytical estimate.
If the project is hold, let involved staff know and confirm sampling date or period, samples preparation and analysis.

2- Analysis Request
If possible, before doing your sampling (some has to be immediately treated while others can be conserved), contact the technical head in order to ask his availability as well as the equipment ones and analysis delay.
Samples preparations are made by the requester (filtration, conditioning, drying, …) and only the analysis will be done by CS CAE staff. Imperatively fill the request form in otherwise analysis will not be done. While analysis returned, CS CAE will give a period of 4 weeks at the requestor to verify his/her data. Over the deadline, CS CAE will flush aliquots out.

3- Using CS CAE’s analytical tools by a trained scientist
Only a trained and skilled scientist by the responsible for the equipment will be able to work in full autonomy.
Booked the machine by subscribing on the notebook near the machine.
The D-day, you can run your analysis by following the hygiene and safety rules of laboratory.
Do not forget to fill the tracking sheet (number of analysis, parameters, running time, …).
And do not forget to clean the machine and laboratory after you.

4- Analytical cost
CS CAE budget is indispensable for machines’ maintenance. Basic consumables (gloves, tips, diluent, acids, basic media, …) are furnished.
Only specific consumables are in charge of the requester (tin basket, specific column, specific media for culture,). A more important financial support can be requested if a big number of samples are analyzed.
Punctual financial support can be asked in case of important breakdown of machine.

5- Sign of recognition of CS CAE’s involvement in samples analysis and/or project research
In function of the CS CAE’s involvement and the workload request to the staff. CS CAE will ask sign of recognition from requesters either by naming CS CAE in acknowledgments or by including staff as co-author.