PICS STRAS

STAINING AND TRACKING SPONGE CELLS TO DESCRIBE MORPHOGENETIC PROCESSES (STRAS)
INTERNATIONAL PROGRAMS FOR SCIENTIFIC COOPERATION (PICS CNRS)

Description

PICS STRAS

Gene content of sponges was characterized by transcriptomic or genomic approaches. Surprisingly, despite their simplicity sponges contain most of the developmental gene families present in bilaterians. The next step to be reached is now to understand how similar genetic toolboxes can result in widely dissimilar bodyplan organization, dynamics and life histories. To answer this question, three main axes of research have to be undertaken : 1) sequencing more whole genomes ; 2) conducting much more gene expression studies (available data remain sparse) ; 3) developing reproducible knock down protocols ; 4) reinterpreting sponge cell structure and mechanisms with nowadays tools.
The 4 partners are interested and involved in these four aspects, but the present project focuses on point 4.The present knowledge of sponge cell biology mainly relies on classical (static) observations, which fail to provide a dynamical description of cellular behaviors and mechanisms, essential to decipher morphogenetic processes.
Much remains to be tested in terms of cell specific staining and tracking to understand which key cellular processes are involved in sponge morphogenesis and to evaluate the so-called “lability” of sponge cells. We propose to join our efforts and skills to make substantial advances in these techniques to evaluate the implications of three primordial cellular mechanisms : cell death, cell proliferation/differentiation and mesenchymal-epithelial/epithelial-mesenchymal transitions (MET/EMT). We will perform these comparisons in 3 species with different features : 1 filtering Demosponge Amphimedon queenslandica, 1 carnivorous sponge (devoid of aquiferous system) Lycopodina hypogea, 1 Homoscleromorph sponge Oscarella lobularis.

We aim at :
Aim 1 : Evaluating the relative stemness of sponge cell types
Aim 2 : Evaluating the relative involvement of EMT/MET during regeneration
Aim 3 : Evaluating the involvement of apoptotic events during regeneration

Aim 1

Aim 1 : Evaluating the relative stemness of sponge cell types

Aim 2

Aim 2 : Evaluating the relative involvement of EMT/MET during regeneration

Aim 3

Aim 3 : Evaluating the involvement of apoptotic events during regeneration


Partners

Marine Genomic Laboratory - Institut Méditerranéen de la Biodiversité et d’Ecologie marine et continentale - Institut des Sciences de l’Évolution de Montpellier - Institut de Biologie du Développement de Marseille

Financial partner
PICS STRAS is supported by CNRS